Characterization of Iranian Isolates of Canine Parvovirus in Fecal Samples Using Polymerase Chain Reaction Assay
Authors: not saved
Abstract:
Despite the widespread prevalence of canine parvovirus disease (CPV) in Iranian dog population,molecular diagnosis of CPV variants, and investigation of the trends of its genetic changes is a new effort. Inthis study 50 samples from dogs suspicious of infection with clinical signs of diarrhea and vomiting, and 25samples from dogs suspected of infection with general symptoms such as depression and anorexia werecollected from dogs presented to the veterinary clinic of Shiraz University, Shiraz, Iran. Viral DNA wasextracted from feces. Three specific pairs of primers, P2, Pab, and Pb, were used in a PCR assay for differential diagnosis of the virus type. Pab primer pairs detect the new type-strains, CPV-2a and 2b. Theprimer pairs P2 and Pb detect CPV types 2 and 2b, respectively. Our results showed that 44 individualswith clinical signs of diarrhea and vomiting were positive for CPV-2. 39 individuals (89%) were positive forCPV-2b and 5 individuals (11%) for CPV-2a. Therefore, the CPV-2b was identified as the predominantvirus type. All dogs without symptoms of diarrhea and vomiting were CPV-negative. The relationship ofbreed, age and sex with PCR results was not significant (P>0.05). For the first time in the country, thecausative agent of CPV-2 was identified, and presence of new antigenic variants, CPV-2a and CPV-2b wasconfirmed.
similar resources
Identification of Pentatrichomonas hominis in feline fecal samples by polymerase chain reaction assay.
Pentatrichomonas hominis is considered to be a commensal protozoan of the vertebrate digestive tract. On the basis of light microscopic examination of feces, some investigators presumptively identified P. hominis as a causative agent of feline diarrhea. However, molecular identification of P. hominis infection in the cat has not been reported. Another trichomonad, Tritrichomonas foetus, is reco...
full textDetection of Different Iranian Isolates of Bovine Herpes Virus Type-I (BHV-I) Using Polymerase Chain Reaction
full text
Detection of Plasmodium falciparum Directly from Blood Samples Using the Polymerase Chain Reaction
full text
Molecular detection of Ehrlichia spp. in blood samples of dogs in southern Iran using polymerase chain reaction
Ehrlichiosis is a zoonotic disease which has been reported from some regions of Iran. This study was aimed to determine the presence and prevalence of ehrlichiosis in suspected dogs referred to the Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran using polymerase chain reaction(PCR). Blood samples were collected from 98 suspected dogs with at least one of the five following findi...
full textOptimization of a species-specific polymerase chain reaction assay for identification of Pentatrichomonas hominis in canine fecal specimens.
OBJECTIVE To determine the optimum reaction conditions and detection limits of PCR assay for identification of Pentatrichomonas hominis in DNA extracted from canine feces. SAMPLE POPULATION DNA extracted from feces of 4 dogs with diarrhea from which trichomonads were observed, 81 dogs that had feces submitted to a diagnostic laboratory, and 19 dogs residing in a laboratory animal facility. ...
full textPolymerase chain reaction assay targeting nox gene for rapid identification of Brachyspira canis in dogs
Genus Brachyspira,as Gram negative anaerobic bacteria, colonize in dogs intestine. The aim of the current study was to determine the prevalence of Brachyspira spp. for the first time in Iran and rapid identification of Brachyspira spp. in dogs by a new designment of a species-specific primer set for B. canis. One hundred fifty-one fecal samples were obtained ...
full textMy Resources
Journal title
volume 9 issue 1
pages 63- 68
publication date 2011-01-01
By following a journal you will be notified via email when a new issue of this journal is published.
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023